Isolation and Identification of Sodium Fluoroacetate Degrading Bacteria from Caprine Rumen in Brazil
نویسندگان
چکیده
The objective of this paper was to report the isolation of two fluoroacetate degrading bacteria from the rumen of goats. The animals were adult goats, males, crossbred, with rumen fistula, fed with hay, and native pasture. The rumen fluid was obtained through the rumen fistula and immediately was inoculated 100 μL in mineral medium added with 20 mmol L(-1) sodium fluoroacetate (SF), incubated at 39°C in an orbital shaker. Pseudomonas fluorescens (strain DSM 8341) was used as positive control for fluoroacetate dehalogenase activity. Two isolates were identified by 16S rRNA gene sequencing as Pigmentiphaga kullae (ECPB08) and Ancylobacter dichloromethanicus (ECPB09). These bacteria degraded sodium fluoroacetate, releasing 20 mmol L(-1) of fluoride ion after 32 hours of incubation in Brunner medium containing 20 mmol L(-1) of SF. There are no previous reports of fluoroacetate dehalogenase activity for P. kullae and A. dichloromethanicus. Control measures to prevent plant intoxication, including use of fences, herbicides, or other methods of eliminating poisonous plants, have been unsuccessful to avoid poisoning by fluoroacetate containing plants in Brazil. In this way, P. kullae and A. dichloromethanicus may be used to colonize the rumen of susceptible animals to avoid intoxication by fluoroacetate containing plants.
منابع مشابه
Defluorination of Sodium Fluoroacetate by Bacteria from Soil and Plants in Brazil
The aim of this work was to isolate and identify bacteria able to degrade sodium fluoroacetate from soil and plant samples collected in areas where the fluoroacetate-containing plants Mascagnia rigida and Palicourea aenofusca are found. The samples were cultivated in mineral medium added with 20 mmol L(-1) sodium fluoroacetate. Seven isolates were identified by 16S rRNA gene sequencing as Paeni...
متن کاملIsolation and identification of crude oil degrading bacteria from mangrove forests of Khamir and Minab ports located at north of Persian Gulf
Mangrove forests are coastal ecosystems that found worldwide in tropical and subtropical zones. Oil spill causes damage to these marine ecosystems. The objective of this study is study the oil-degrading bacteria in some mangrove forests that located at Persian Gulf. In this study sediments and plant materials were collected from mangrove forests at Persian Gulf (Minab and Khamir ports). Oil-deg...
متن کاملIsolation and Identification of Carcinogen Acenaphthene-Degrading Endemic Bacteria from Crude Oil Contaminated Soils around Abadan Refinery
Background and Objective: PAHs are non-polar organic compounds consisting of two or more fused benzene multi-rings. Among these compounds, acenaphthene is a multi-ring hydrocarbon that occurs abundantly in nature. Use of microorganisms to clean the contaminations of soil can be cheap and effective. The most important acenaphthene-degrading bacteria are pseudomonas, micrococcus, and Bacillus. Th...
متن کاملIsolation and identification of phenol degrading bacteria from Kor river and their growth kinetics assay
Background : Phenol and it’s derivatives were removed by physicochemical methods in the past but nowadays biological treatment has priority. Bacteria with the rapid proliferation in the presence of phenol and it’s derivatives can remove them in a good manner. The aim of this project is isolation and identification of bacteria that degrade phenol from water and sediment of Kor river and also inv...
متن کاملIsolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils
Background: Organic agricultural pesticides are so stable in ecosystems and biosphere. These compounds affect soil conditions and agricultural products. The aim of this study was isolation of diazinon-degrading bacteria from Kerman pistachio orchards, Southeastern Iran. Methods: Diazinon-degrading bacteria were enriched in Bushnell-Hass medium. Identification and sequencing of prevalent degr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
دوره 2012 شماره
صفحات -
تاریخ انتشار 2012